Figure 4
From: Polyphenols act synergistically with doxorubicin and etoposide in leukaemia cell lines
The effect of doxorubicin (DOX) and etoposide (ETP) on caspases 8 and 9 activity when used in combination with quercetin (QUE), apigenin (AP), emodin (EMO), rhein (RH) or cis-stilbene (CIS) in lymphoid leukaemia cell lines (Jurkat and CCRF-CEM); and when used in combination with QUE or AP in myeloid leukaemia cell lines (THP-1 and KG-1a). This was evaluated by Caspases-Glo Luminescent 8 and 9 Assays. Cells were treated with DOX or ETP and polyphenols alone and in combination for 24 h using their lowest-significant doses (LSD). Data were normalised to the vehicle control, which was assigned a 0 RLU. The data were expressed as medians with ranges (n=4). The black bars show the vehicle controls and treatments alone; the coloured bars indicate significant additive effects in brown, synergistic effects in yellow, competitive antagonistic effects in purple and antagonistic effects in pink. Statistical significance was set at P⩽0.05 compared with vehicle control, drugs alone and expected values of individual drugs effects.
