Figure 7
AACOCF3, a cPLA2-specific inhibitor reverses EtOH and/or HIV-1-mediated inflammatory responses. IL-1β (a), TNF-α (b), COX2 (c) and CYP2E1 (d), mRNA levels were measured in astrocytes exposed to AACOCF3 1 h, followed by continuous treatment with EtOH±HIV-1 for 24 h. In parallel experiments, whole-cell protein lysates were collected and resolved by SDS-PAGE, transferred and immunoblotted for COX2 and CYP2E1. GAPDH was used as normalizing control. Representative western blots with densitometry as fold changes to controls are shown (Figure 7e and f). Three independent astrocyte donors were analyzed in multiple replicates and cumulative data normalized to controls are shown as fold changes (a–d). Statistical analyses were performed using one-way ANOVA with Bonferroni post hoc test for multiple comparisons (*P<0.05, **P<0.01, ***P<0.001, #P<0.05 and ##P<0.01). # Represents statistical significance for comparisons±AACOCF3, in respective treatments.
