Figure 4
From: Inhibition of malignant thyroid carcinoma cell proliferation by Ras and galectin-3 inhibitors
Combined treatment with FTS and MCP inhibit ARO thyroid cell tumor growth in a nude mouse model. ARO cells were injected subcutaneously into the flank areas of nude mice; tumor volumes and weights were determined as described in Materials and Methods. (a) Tumor volume in FTS, MCP, FTS+MCP-treated and control mice as a function of time; points—mean; bars—S.E.M.; C—control, F-FTS, M-MCP, F+M-FTS+MCP. (b–d) After 35 days, mice were killed and tumors were weighed and then homogenized for immunoblot analysis. (b) Tumor weight of control (black bar), FTS (gray bar), MCP (upward diagonal bar) and FTS+MCP (white bar)-treated mice. (c) Representative Immunoblots of p-ERK and Gal-3 normalized to actin and K-Ras-GTP, visualized by ECL. (d) Apparent levels of Galectin-3, p-ERK, and K-Ras-GTP determined by densitometry (a.u.) of the immunoblots. Results are presented as means±SEM; one-way ANOVA analysis revealed significant differences between tumor volumes, tumor weights and protein levels; post hoc analysis was performed by the HSD test and Fisher's LSD test. *P<0.05, **P<0.01, ***P<0.001; FTS, S-farnesylthiosalicylic acid; MCP, modified citrus pectin.
