Figure 2 | Cell Death Discovery

Figure 2

From: A dual role for Caspase8 and NF-κB interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival

Figure 2

IKKβ inhibition downregulates NF-κB signaling and caspase activation after TNFα stimulation. (a) Ovcar3 cells expressing control or Caspase8 shRNA were transduced with NF-κB luciferase reporter. NF-κB signaling in was measured after 18 h treatment with TNFα (10 ng/ml) and/or IKKβ inhibitor (2.5 μM). Data are arbitrary luciferase units normalized to XTT and are represented as mean±S.E.M.; *P<0.05 based on t-test. (b) mRNA expression of Caspase8 and NF-κB target genes cFLIP, CLDN1, CXCL1, CXCL2 and IL-8 was measured by quantitative RT-PCR in Ovcar3 cells, expressing either control or Caspase8 shRNA. Expression was normalized to that of GAPDH, and expressed as mean±S.E.M. of triplicate experiments. *P<0.05 based on t-test.

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