Figure 5
IL-16C release upon treatments inducing secondary necrosis of neutrophils. (a) Neutrophils were irradiated with 0, 200, 800 and 1600 mJ/cm2 UV light (256 nm). After 6 h of incubation at 37 °C, the supernatants were analyzed with ELISA for released IL-16 and MIF. Neutrophil viability was assessed with flow cytometry using Annexin-V-FLUOS and PI staining. Bars represent the means±S.E.M. of three independent experiments (*P<0.05; **P<0.01; ***P<0.001, ****P<0.0001 compared with the 0-h sample by one-way ANOVA followed by Holm Sidak multiple comparison correction). Individual data points are shown as black dots. (b) Neutrophils were infected with A. phagocytophilum (A. phago) for 6 at 37 °C. The supernatants were analyzed with ELISA for released IL-16 and MIF, and neutrophil viability was assessed with flow cytometry using Annexin-V-FLUOS and PI staining. Bars represent the means±S.E.M. of four independent experiments (*P<0.05; **P<0.01; ***P<0.001, ****P<0.0001 compared with the uninfected control by paired t-test). Individual data points are shown as black dots.
