Figure 2
From: p53 Configures the G2/M arrest response of nucleostemin-deficient cells
NS depletion triggers G2/M arrest in both p53wt and p53ko MEF cells.(a) MEF cells derived from NSflx/flx or inNScko embryos in the p53wt or p53ko background were treated with DMSO (gray bars) or TAM (0.1 μM, black bars) for 4 days at P2 and analyzed by propidium iodide (PI)-labeled flow cytometry. The averages for p53wt cells (n=9) and p53ko cells (n=4) were shown in (a1) and (a2), respectively. (b) The percentages of prophase, metaphase, anaphase, and telophase cells were quantified by anti-pH3 staining in p53wt (b1) and p53ko (b2) MEF cells from eight experiments. See Figure 1 for description regarding error bars and asterisks.
