Figure 6
From: p53 Configures the G2/M arrest response of nucleostemin-deficient cells
Molecular responses of p53wt and p53ko cancer cells to NS depletion. HCT116-8 (p53wt) and HCT116-2 (p53ko) cells were treated with siScr or siNS for 15 h and collected for analysis after 2 days. (a) The mRNA levels of p53-downstream genes were measured by qRT-PCR assays. (b) The amount of phosphorylated cdc2 (Y15), NS, and p53 in control (siScr) and NS-knockdown (siNS) HCT116-8 and HCT116-2 cells were measured by western blots. See Figure 1 for description regarding error bars and asterisks.
