Figure 3 | Cell Death Discovery

Figure 3

From: Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio

Figure 3

Immunoblot analysis of cell cycle regulatory and apoptosis-related proteins of cell lysates of MCF-7 cells treated with DBME (200 μg/ml) for the indicated time intervals. (a) Cell cycle, Bcl-2 family, p21 proteins. (b) Caspase family, PARP, p53 proteins. (c) Effects of caspase inhibitors on DBME-induced apoptosis in MCF-7 cells. The cells were treated with Z-IETD-fmk, Z-LEHD-fmk and both in the presence of DBME (200 μg/ml) for 48 h and analyzed by Annexin-V–PI staining using flow cytometer. Data are expressed as mean±S.D. (N=3).

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