Figure 3 | Cell Death Discovery

Figure 3

From: AMPK maintains energy homeostasis and survival in cancer cells via regulating p38/PGC-1α-mediated mitochondrial biogenesis

Figure 3

AMPK regulates p38-dependent expression of PGC-1α. (a, b) Immunoblots showing levels of indicated molecules in H1299-EV and H1299-DN cells (a) and in MEFs (b) cultured in 25 or 1 mM glucose with or without 0.5 mM AICAR for 24 h. HSP60 was used as loading control. (c) Relative expression of PGC-1α and COX5b mRNA in H1299-EV and H1299-DN cells grown under the conditions used in a as evaluated by qRT-PCR. (d–f) H1299-EV, H1299-DN, WT-MEF and DKO cells were grown under conditions mentioned in a. Respiratory complex I activity (d), citrate synthase activity (e) and relative levels of ATP (f) were measured as described in Materials and Methods. (g) Immunoblots of indicated molecules in H1299 cells cultured in glucose-limiting conditions with or without 0.5 mM AICAR and 10 μM p38-specific inhibitors, SB203508 and SB220025 for 24 h. Values are represented as mean±S.D. *P<0.05, **P<0.01 and ***P<0.001 denote significant differences between the groups.

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