Figure 5 | Cell Death Discovery

Figure 5

From: AMPK maintains energy homeostasis and survival in cancer cells via regulating p38/PGC-1α-mediated mitochondrial biogenesis

Figure 5

AMPK promotes glycolysis by activating PFK2. (a) H1299 cells were grown in DMEM without glucose and glutamine, and supplemented with 1 mM, 5 mM and 25 mM glucose. Cells were simultaneously treated with 0.5 mM AICAR and/or 10 μM compound C for 24 h. Fresh medium (DMEM containing 25 mM glucose and 10% FBS) was added after 24 h and further grown for indicated time points. Time-dependent glucose utilization in H1299 cells under basal (i), AICAR treated (ii) or in the presence of compound C plus AICAR (iii). (b) Representative photographs of culture medium from H1299 cells grown under above-mentioned conditions for 24 h. (c, d) lactate secretion (c) and glucose utilization (d) in H1299 cells grown under the conditions used in a. (e) Immunoblots showing phosphorylated and basal levels PFK2 in H1299 (EV and DN) cells grown under indicated conditions for 24 h. (f) PFK activity in H1299 (EV and DN) cells grown under indicated conditions for 24 h. (g) Relative glucose utilization in H1299-EV and H1299-DN cells treated with or without AICAR for 36 h. Values are represented as mean±S.D. *P<0.05, **P<0.01 and ***P<0.001 denote significant differences between the groups.

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