Figure 5 | Cell Death Discovery

Figure 5

From: Autophagy mediated by arginine depletion activation of the nutrient sensor GCN2 contributes to interferon-γ-induced malignant transformation of primary bovine mammary epithelial cells

Figure 5

Autophagy is required for IFN-γ-induced BMEC transformation. (a) The expression levels of LC3-II in BMECs treated with IFN-γ (0 or 10 ng/ml) for up to 8 weeks were determined by western blot analysis with specific antibodies as described in the Materials and Methods section. The data represent the mean±S.E.M. of three independent experiments. Error bars are ±S.E.M. One-way ANOVA; *P<0.05; **P<0.01. (b) BMECs were exposed to IFN-γ (10 ng/ml), 3-MA (1 mM) or rapamycin (Rap; 10 nM) alone or in combination. Cells (1250) were grown on soft agar, and colonies were monitored after 3 weeks. The data represent the mean±S.E.M. of three independent experiments. Error bars are ±S.E.M. One-way ANOVA; *P<0.05, **P<0.01. (c) BMECs were exposed to IFN-γ (10 ng/ml), 3-MA (1 mM) or rapamycin (Rap; 10 nM) alone or in combination. The expression levels of LC3-II were analysed using western blot analysis with specific antibodies as described in the Materials and Methods section. The data represent the mean±S.E.M. of three independent experiments. Error bars are ±S.E.M. One-way ANOVA; *P<0.05; **P<0.01.

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