Figure 1
From: The plant defensin NaD1 induces tumor cell death via a non-apoptotic, membranolytic process
NaD1 inhibits growth of mammalian tumor cells over 24 h at subacute concentrations that do not efficiently induce rapid membrane permeabilization. The ability of NaD1 to inhibit growth of MM170, HeLa, Jurkat and U937 cells was determined using the MTT/MTS cell viability assay. Cells were treated with increasing concentrations of NaD1 (1.25, 2.5, 5 and 10 μM) for 24 h, with determination of cell viability calculated as the percentage of 100% viability (untreated control samples). Half-maximal inhibitory concentration (IC50) values (μM) were extrapolated using Excel (annotated by black dashed arrows). Data in (a) is representative of three independent experiments. (b) The release of LDH from mammalian tumor cells following 30 min treatment with NaD1 at subacute concentrations (1.25, 2.5 and 5 μM) was measured via the LDH cytotoxicity assay. Data in (b) are representative of at least two independent experiments, error bars represent S.E.M, n=3.
