Figure 3
From: The plant defensin NaD1 induces tumor cell death via a non-apoptotic, membranolytic process
NaD1-mediated loss of mitochondrial membrane potential in MM170 cells occurs simultaneously to plasma membrane permeabilization. (a) The effect of NaD1 on the mitochondrial membrane potential in MM170 cells was determined by live cell imaging using confocal laser scanning microscopy (CLSM). Cells were pre-treated with MTR before being treated with 1.25 μM NaD1 and imaged at 30 s intervals over 4 h in growth medium containing FITC-dextran (4 kDa). Images of key time points (taken from Supplementary Video S1) display uptake of FITC-dextran and loss of MTR fluorescence in a single cell (white arrows). Relative time is indicated in top left of each panel (h:m:s:ms). Data are representative of three independent experiments. (b) Kinetic analysis of mean fluorescence intensity of FITC-dextran versus MTR fluorescence was plotted for individual cells from 1 min before to 7 min after final baseline reading of FITC-dextran before observable uptake into the cell (Time ‘0’, indicated by red dashed line). Data in (b) represent average fluorescence across all cells. n=27, error bars represent S.E.M.
