Figure 1

Tcf7l1 and LCN2 expression in skin diseases. (a) By immunohistochemistry, Tcf7l1 and LCN2 were stained in normal skin (n=4), seborrheic keratosis (n=4), psoriasis vulgaris (n=5), verrucous epidermal nevus (n=3), pigmented nevus (n=4), Bowenoid papulosis (n=3), BCC (n=4), condyloma acuminatum (n=4) and SCC (Broder’s grade I, n=3). (b) By western blot, the Tcf7l1 and LCN2 proteins were detected individually in all tissue lysates, followed by a quantitation of band intensities using ImageJ. Numbers of 1 to 9 indicate the normal skin and eight diseases shown in (a) in order. *P<0.05, **P<0.01, compared with normal skins accordingly. Data are expressed as mean±S.E.M. Statistical differences were tested with one-way ANOVA followed by a two-tailed t-test. (c) The mRNA expression levels were determined individually, followed by the linear correlation between the Tcf7l1 and LCN2 values (n=34, P<0.05). Linear regression was done for the correlation between the mRNA values of Tcf7l1 and LCN2. Representative images are shown. Scale bar=50 μm.