Figure 3 | Cell Death Discovery

Figure 3

From: Transcription factor 7-like 1 dysregulates keratinocyte differentiation through upregulating lipocalin 2

Figure 3

Tcf7l1 and LCN2 expression in keratinocytes undergoing differentiation. HFKs were cultured in vitro with the addition of calcium (1.2 mM). (a and b) The mRNA levels of Tcf7l1 (a) and LCN2 (b) were determined in HFKs that were harvested at different time points. (c and d) Western blot was performed with protein extracts of HFKs for Tcf7l1 and LCN2 expression (c), followed by quantitation of band intensities with ImageJ (d). Data show mean±S.E.M. of triplicate cultures (n=3). Statistical differences were tested with one-way ANOVA followed by a two-tailed t-test. Representative images are shown. *P<0.05, **P<0.01, compared with the values on day 0 accordingly.

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