Figure 6
The effect of LCN2 inhibition on HFKs. (a and b) By western blot, involucrin and loricrin expression was determined in Tcf7l1-transfected HFKs that were then treated with LCN2 or control siRNA. (c and d) Similarly, involucrin and loricrin were assessed in E6/E7-transfected HFKs that were treated with neutralizing anti-LCN2 antibody or isotype control (2 μg/ml, 2 days). (e and f) By immunofluorescence (e) and western blot (f), keratin 1 was detected in HFKs that were transfected with Tcf7l1 vector and then LCN2 or control siRNA. (g) HFKs were pre-transfected with Tcf7l1 vector, and then transfected with LCN2 siRNA. Cell apoptosis was quantitated by the CaspaTag caspase 3, 7 method. Data show mean±S.E.M. of triplicate cultures (n=3). Statistical differences were tested with one-way ANOVA followed by a two-tailed t-test. Representative images are shown. Scale bar=10 μm. *P<0.05; **P<0.01.
