Figure 7
The effect of MMP-2 on LCN2 expression in cells. (a) By western blot, the expression of Tcf7l1, MMP-2 and MMP-9 was determined in SCC-13 cells and Tcf7l1- or E6/E7-transfected HFKs. (b) The ratios of MMP-2 to MMP-9 were calculated by using the relative intensity values from western blot. *P<0.05, **P<0.01, compared with the control HFK group. (c) By western blot, the LCN2 protein was detected in the HFKs that were transfected with Tcf7l1-expressing lentiviral vector or MMP-2/control siRNA. *P<0.05, compared with the nontreated group; Δp<0.05, compared with Tcf7l1 transfection alone group; †P<0.05, compared with control siRNA group. (d) The MMP-2 and MMP-9 proteins were blotted in cell lysates that were precipitated by anti-LCN2 IgG-conjugated protein IgG agarose. The ratios mean MMP-2 to MMP-9 values. Data show mean±S.E.M. of triplicate cultures (n=3). Statistical differences were tested with one-way ANOVA followed by a two-tailed t-test.
