Figure 4 | Cell Death Discovery

Figure 4

From: Mitochondria mediates caspase-dependent and independent retinal cell death in Staphylococcus aureus endophthalmitis

Figure 4

S. aureus induces mitochondrial translocation of Bax in retinal Müller glia. MIO-M1 cells were left uninfected (control) or challenged with S. aureus RN6390 (multiplicity of infection, 10 : 1) for the indicated time points. Cells were stained with MitoTracker red (Mito Red) followed by immunostaining for Bax and observed under confocal microscope (a). In another experiment, S. aureus-challenged MIO-M1 cells were subjected to subcellular fractionation followed by western blot analysis for Bax (b). Cox 4 and β-actin antibodies were used as protein loading controls for mitochondrial and cytoplasmic fractions, respectively. Results are representative of two independent experiments.

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