Figure 1
From: NGF sensitizes TrkA SH-SY5Y neuroblastoma cells to TRAIL-induced apoptosis
NGF sensitizes TrkA SH-SY5Y cells to TRAIL-induced apoptosis. (a) Representative phase contrast and fluorescent micrographs (bar=100 μM) and (b) histograms demonstrating the marked induction of TrkA SH-SY5Y but not NT or pcDNA SH-SY5Y cell death following 16 h of incubation with NGF (100 ng/ml) plus TRAIL (200 ng/ml), compared with the lack of cell death in all three cell lines following overnight (16 h) incubation with either NGF (100 ng/ml) or TRAIL (200 ng/ml) alone. Results are displayed as the mean percentage (%) of alive (white) and dead (black) cells (±S.D.) in three independent experiments each performed in duplicate (*statistical significance). (c) Representative phase contrast micrographs demonstrating the effect of NGF plus TRAIL on the morphology of surviving TrkA SH-SY5Y cells. (d) Histogram demonstrating dose-dependent induction of TrkA SH-SY5Y cell death by TRAIL (10–500 ng/ml) in the presence but not absence of NGF (100 ng/m). Results are displayed as the mean percentage (%) (±S.D.) of alive (white) and dead (black) cells in three independent experiments each performed in duplicate. (e) Representative ethidium bromide stained agarose gel demonstrating laddering of DNA purified from TrkA SH-SY5Y cells treated for 16 h with NGF (100 ng/ml) plus TRAIL (200 ng/ml) but not from TrkA SH-SY5Y cells treated with either NGF (100 ng/ml) or TRAIL (200 ng/ml) alone.
