Figure 6
From: Modulation of therapy-induced senescence by reactive lipid aldehydes
Lipid aldehyde scavenger HYD prevents the onset of AS. (a) Chemical structures of NAC, a general free-radical scavenging antioxidant, LPA, a general lipid antioxidant, and HYD HCl (HYD), an aldehyde scavenger. (b–e) Senescence assays were conducted for etoposide-treated cells that had been pretreated with compounds for 2 h, including (b) DMSO (0.5%), (c) NAC (1 mM), (d) LPA (5 mM) and (e) HYD (1 mM). Strikingly, HYD completely suppressed etoposide-induced senescence. (f) SI for each compound treatment shown in (b–e) indicating suppression of AS after etoposide by lipid antioxidants and aldehyde scavengers. (g) Median ROS signal for each compound treatment shown in (b–e). Notably, LPA and HYD increased ROS in etoposide-treated cells despite suppressing AS. (h) HYD blocked induction of senescence after IR or treatment with topoisomerase inhibitors.
