Figure 2 | Cell Death Discovery

Figure 2

From: Caspase-2 resides in the mitochondria and mediates apoptosis directly from the mitochondrial compartment

Figure 2

Mitochondrial caspase-2 can trigger apoptosis in cell-free systems. Rat liver nuclei and Xenopus oocyte cytosol were mixed with purified mitochondria from (a) WT or Casp2−/− brain or (b) WT or Casp2−/− liver and incubated at 37˚C for 4 h. Shown are the apoptotic potency ratios (calculated as the percent ratio of apoptotic nuclei in sample to buffer) from three independent experiments. (c) Representative images of liver nuclei mixed with cytoplasmic and mitochondrial fractions from either WT or Casp2−/− mice at 4 h. Upper panels are magnified images of the insets indicated in the respective lower panels. Panels 1–4 contain WT cytoplasmic fraction whereas panels 5–8 contain Casp2−/− cytoplasmic fraction. (d) Apoptotic potency ratios of the different combinations at the indicated time points.

Back to article page