Figure 3

Mitochondrial caspase-2 induces cytochrome c release. Xenopus oocyte cytosol was mixed with purified mitochondria from either WT or Casp2−/− brain, centrifuged to separate mitochondrial and cytosol fractions and immunoblotted for cytochrome c (cyt c). (a) Representative blot from three independent experiments; Rec denotes recombinant cyt c loaded as a positive control. (b) Densitometry analysis after normalization to actin.