Figure 2 | Cell Death Discovery

Figure 2

From: BUB1 and SURVIVIN proteins are not degraded after a prolonged mitosis and accumulate in the nuclei of HCT116 cells

Figure 2

Consequences of mitotic arrest in HCT116 and HT29 cells. After the cells were synchronized by thymidine block (18 h), they were exposed to paclitaxel (100 nM). (a) Polyploid cells that escape from mitosis and enter a new cell cycle (DNA content>4) are evident after 24 h of treatment. (b) Simultaneously, the percentage of dead cells increases (DNA content<2N). (c) The number of polyploid HT29 cells is not significant after 48 h of treatment in accordance with longer arrest in this cell line. (d) After HT29 cells are treated with paclitaxel for 36 h, the percentage of dead cells increases but remains near 10% until 48 h.

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