Figure 6
Heterogeneity in cell fate and morphology after a mitotic arrest. HCT116-H2BDsRed cells were synchronized by thymidine block, treated with paclitaxel (100 nM) and analyzed by time lapse. (a) Cell enters mitosis (arrow) at 12.3 h and exits mitosis at 33.6 h (cytokinesis-like). The cell is reorganized at 42.9 h and is maintained until the end of the time-lapse imaging (50.6 h). (b) Cell dying in mitosis (arrow). Chromosome condensation is evident at 25.3 h; at 25.6 h, membrane blebbing is initiated, and the cell dies. (c) Cell exits mitosis and divides into fragments (arrow). Cell enters mitosis at 28.4 h. Membrane is protruding at 31.3 h, and a cytokinesis-like state with membrane extensions is evident at 39.9 h (arrows). The cell is reorganized into three fragments (arrows) at 42.9 h. (d) Larger cell forms after mitotic arrest (arrow). Chromosome condensation begins at 12.1 h, chromosome decondensation begins at 13.1 h, and the cell is reformed into a larger cell at 31.6 h. Scale bar equal to 20 μm.
