Figure 8
Activation of inflammation in vitro and in vivo and its prevention by chromatin neutralizing/degrading agents. (a) Time-course analysis of activation of inflammatory cytokines following co-cultivation of NIH3T3 cells with dead Jurkat cells as detected by indirect immunofluorescence. The experiments were done in duplicates at each time point and the mean values were plotted in the graph. (b) Representative images showing activation of inflammatory cytokines in NIH3T3 cells following co-cultivation with dead and live Jurkat cells with NIH3T3 cells at 6 h and their prevention by CNPs, DNase I and R-Cu. (c) Quantitative analysis of images shown in b. Activation of NFκB (left upper panel), IL-6 (right upper panel), IFNγ (left lower panel) and TNFα (right lower panel). The experiments were done in duplicate and 500 cells were analyzed in each case to determine mean fluorescence intensity (MFI). Mean (±S.E.) values are depicted in the histograms and the results were compared by Student’s t-test. ****P<0.0001. (d–g) Representative images showing activation of inflammatory cytokines at 72 h in vital organs of mice following intravenous injection of dead and live Jurkat cells (1×105) as detected by indirect immunofluorescence. (h) Quantitative analysis of expression of NFκB following intravenous injection of dead and live B16-F10 cells (1×105) and their prevention by CNPs, DNase I and R-Cu. The experiment was done in duplicate and 1000 cells were analyzed in each case to determine MFI. Mean (±S.E.) values are depicted in the histograms and the results were compared by Student’s t-test. **P<0.01, ***P<0.001.
