Figure 2 | Cell Death Discovery

Figure 2

From: Escherichia coli O157:H7 suppresses host autophagy and promotes epithelial adhesion via Tir-mediated and cAMP-independent activation of protein kinase A

Figure 2

E. coli O157:H7 inhibits autophagic flux. (a) Autophagic degradation activity is inhibited by EDL933 WT strain as indicated by altered LC3B and p62 protein levels 4 h post infection. CQ: cells were treated with 20 μM CQ during the course of infection. (b and c) Microscopic analysis of HT-29 cells transfected with tfLC3 (tandem fluorescent tagged LC3B) plasmids 4 h post infection. mRFP: Red puncta represent autophagolysosome that is late stage of autophagy tagged with mRFP; mRFP/EGFP: Yellow puncta represent autophagosome that is double tagged with both mRFP and EGFP; Control: HT-29 cells with tfLC3 without infection; E. coli O157:H7: HT-29 cells with tfLC3 challenged with EDL933 WT strain. Images were taken at ×400 magnification. Means±S.E.M.; n=4. **P<0.01.

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