Figure 2
Myeloid cells undergo apoptosis/necrosis during the course of infection with Ft. Total lung cells were isolated from C57BL/6 mice infected with 103 CFU of Ft LVS (a and b) or 20 CFU of SchuS4 (c and d) at day 3 p.i. The cells were stained with TUNEL (a and c) or 7-aminoactinomycin D (7-AAD) (b and d) to quantify the percentage of myeloid cells undergoing apoptosis and necrosis. The lungs of Ft SchuS4-infected mice were not perfused before isolation of cells as to minimize potential liquid dispersal of select agent. Data are presented as the mean±S.E.M. from three independent experiments (n=6 mice per group or 18 mice total). **P<0.01, and ***P<0.001.
