Figure 4

Electron microscopic analysis of leukocytes in normal and Ft LVS-infected lung. (a) Normal PMN isolated from sham-inoculated lung showing multilobed nucleus with normal distribution of chromatin structures. (b) Activated PMN exhibiting signs of apoptosis with condensed/marginated nuclear chromatin (N) and cytoplasmic vacuoles (v). (c) Necrotic cell (Nec) showing grossly swollen mitochondria being released into the extracellular space (arrows). Red blood cell, RBC. (d) Necrotic cell (Nec) being recognized by adjacent macrophage (Mac). Free mitochondrion (arrow) outside the cell appears to be phagocytized by a macrophage.