Figure 7
Ft induces necroptotic cell death in macrophages. (a) Wild-type (WT) and RIP1/3−/− BMDMs were infected with Ft LVS at a multiplicity of infection (MOI) of 100 and lactate dehydrogenase (LDH) release was measured at the indicated time points. (b) WT BMDMs either were left untreated or were treated with dimethyl sulfoxide (DMSO) alone or necrostatin-1 (20 μM) and LDH release was measured at the indicated time points. (c) WT BMDMs were treated for 24 h with medium alone or medium containing 5 μM staurosporine (to induce apoptosis), or LPS+zVAD (to induce necroptosis) or with Ft alone. Dead cells were collected, counted, and resuspended in fresh medium and were applied to naïve BMDMs at a 2 : 1 ratio. After 2 h, cells were washed with cold phosphate-buffered saline (PBS) and the fluorescence intensity was quantified using a plate reader. Data are presented as the mean±S.E.M. from three independent experiments. *P<0.05, **P<0.01. All results shown were subjected to one-way analysis of variance (ANOVA) with Bonferroni’s post-test.
