Figure 3

Transcriptome analysis of HCC cells in response to aspirin and sorafenib. (a) The heatmap shows a panel of HCC dysregulated genes significantly enriched in combination treatment of aspirin and sorafenib in Hep3B (i) and HuH7 (ii) cells. (b) The dot plot showed the synergistic blockade of ACSL4 and induction of GADD45B by aspirin and sorafenib combination in Hep3B (i) and HuH7 (ii) cells by microarray analysis. (c and d) The real-time RT-qPCR analysis demonstrated a synergistic blockade of ACSL4 (i) and induction of GADD45B (ii) by the aspirin and sorafenib combination but not in single drug treatment in Hep3B (c) and HuH7 (d) cells. *P<0.05.