Figure 3 | Cell Death Discovery

Figure 3

From: ATF3 reduces migration capacity by regulation of matrix metalloproteinases via NFκB and STAT3 inhibition in glioblastoma

Figure 3

Efficiency of ATF3 knockdown and overexpression in U87 cells. U87 cells were stably infected with lentiviral particles containing shRNA for ATF3 or ATF3 and controls. (a) Target gene expression was measured by qRT-PCR and normalized to controls pGIPZ (shATF3) or to pLOC (ATF3) set to 1. In addition, cells were treated with 15 μM JS-K for 48 h to induce ATF3 expression. ATF3 was inducible in uninfected U87 and in controls pGIPZ and pLOC. (b) The protein level of ATF3 was examined by western blot analysis in overexpressing and knockdown cells. GAPDH was used as loading control. Figures are representative for three independent experiments.

Back to article page