Figure 7

PCR of migration-related genes was performed to assess gene expression alterations underlying the changes in migration capacity. U87 cells (a) and primary IC cells (b) were treated with JS-K (0.5–3.0 μM) for 48 h and tested for mmp2, mmp7, mmp9 and timp 1–4 expression. Cells exhibiting knockdown or overexpression of ATF3 were additionally tested for expression of mmp1 and mmp3. GAPDH was used as loading control. Figures show representative images of triplicates.