Figure 1

Prdx6 decline in aging triggered increased oxidative load, restricted cell growth and accumulation of LPO contents in aging and glaucomatous (GL) TM cells. (a, b) Cultured primary TM cells at passage (p) 3 to 4 derived from human subjects of variable ages were collected and processed for expression assays. (a) qPCR showing decline of Prdx6 transcript with age and glaucoma. Total RNA was isolated and submitted to qPCR analysis using probes specific to Prdx6 gene. (b) Cells were collected and cellular extracts were extracted and immunoblotted with anti-Prdx6 antibody as shown. Lower panel, histograms show densitometric analysis of protein bands of Prdx6. (c) Increase of ROS in normal TM cells of variable ages, and dramatic elevation in GL TM cells. Histogram values reflect mean±S.D. of three independent experiments. Asterisks indicate P-values from t-test comparisons to control (*P<0.001). (d) Histograms showing increased levels of LPO contents in aging TM cells and GL TM cells. Values reflect mean±S.D. of three independent experiments. Asterisks indicate P-values from t-test comparisons to control. (*P<0.001). (e) Age-dependent restricted cell growth in normal and GL TM cells. Cultured TM cells of variable ages and GL TM cells were subjected to BrdU assay for 24 h. Cell growth was documented in the form of histograms as indicated. Asterisk indicates significant difference (*P<0.001).