Figure 3 | Cell Death Discovery

Figure 3

From: Mitochondrial DNA haplotypes induce differential patterns of DNA methylation that result in differential chromosomal gene expression patterns

Figure 3

Replicative efficiency of divergent mtDNA ES lines. CC9mus, CC9spretus, CC9dunni and CC9pahari ES cells were induced to differentiate into neural lineages over 21 days and were assessed at days 0 (a), 3 (b), 12 (c) and 21 (d) of differentiation for mtDNA copy number and ratios of 5mC and 5hmC. MtDNA copy number was assessed by real-time PCR. Levels of enrichment for 5mC and 5hmC were assessed by MeDIP using antibodies against 5mC and 5hmC, and real-time PCR across exon 2 of PolgA. The data are expressed as a ratio of mtDNA copy against 5mC/5hmC, where 5mC and 5hmC are indicative of DNA methylation and DNA demethylation, respectively. **P<0.01; ***P<0.001; ****P<0.0001.

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