Figure 4 | Cell Death Discovery

Figure 4

From: Specific mutations in the D1–D2 linker region of VCP/p97 enhance ATPase activity and confer resistance to VCP inhibitors

Figure 4

Mutations at codon 470 enhance VCP activity and attenuate VCP inhibitors function. (a) Time-dependent experiment with wild-type (WT) VCP/p97 and two E470 mutants to determine their specific ATPase activities. Specific ATPase activity of VCP proteins presented in the figure was measured in nmol/min/nmol. (b–d) Titration curves of CB-5083, NMS-873 and ML240 for WT, E470K and E470D VCP proteins. IC50 (μM) was calculated using GraphPad Prism with the equation [log(agonist) versus response−Variable slope (four parameters)].

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