Figure 6 | Cell Death Discovery

Figure 6

From: 5-Aminoimidazole-4-carboxamide ribonucleoside-induced autophagy flux during differentiation of monocytic leukemia cells

Figure 6

The effect of simultaneous decrease in hVPS34 and Atg7 protein levels on the expression of differentiation markers. Cells were transfected with siRNA against hVPS34 and Atg7 (four sequences) and respective non-targeting siRNA was used as a negative control. AICAR (0.5 mM), ATRA (1 μM), metformin (15 mM) or PMA (50 nM) were added 24 h after transfection. Total cell lysates were isolated 48 h after addition of agents and analyzed by western blot. The expression of differentiation markers was analyzed by flow cytometry 48 h after addition of agents. Western blot analyses and data obtained by flow cytometry are shown for each of the three independent experiments (Exp 1–3).

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