Figure 1 | Cell Death Discovery

Figure 1

From: Akt1 and Akt3 but not Akt2 through interaction with DNA-PKcs stimulate proliferation and post-irradiation cell survival of K-RAS-mutated cancer cells

Figure 1

Overexpression of Akt isoforms and DNA-PKcs in A549 or HEK293T cells. Expression of Akt and DNA-PKcs fusion proteins. (a) Schematic overview of AKT1, AKT2 and AKT3 constructs fused to mCherry as well as the constructs expressing different subdomains of DNA-PKs fused to eGFP. (b) A549 cells were transfected with the indicated constructs. Twenty-four hours after the transfection, fluorescent images were acquired. Cells expressing mCherry or eGFP alone served as controls. Scale bars: 20 μm. (c) HEK293T cells were transfected with the indicated constructs. Twenty-four hours after the transfection, the cells were lysed and eGFP fusion proteins were precipitated using the GFP-Trap. Whole-cell lysates (mCherry) and the bound fractions (eGFP) were subjected to SDS-PAGE followed by western blot analysis using antibodies specific for mCherry or eGFP.

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