Figure 4 | Cell Death Discovery

Figure 4

From: Akt1 and Akt3 but not Akt2 through interaction with DNA-PKcs stimulate proliferation and post-irradiation cell survival of K-RAS-mutated cancer cells

Figure 4

Akt1 and Akt3 but not Akt2 stimulate repair of IR-induced DSBs. (a) A549 cells were grown on glass slides and transfected with 50 nM of control-siRNA (ctrl-si), AKT1-siRNA (AKT1-si), AKT2-siRNA (AKT2-si) or AKT3-siRNA (AKT3-si). Forty-eight hours to 72 hours after the transfection, protein samples were isolated and the knockdown efficiency was tested by immunoblotting. In parallel to protein isolation, additional cells were irradiated with 4 Gy, and 24 h after irradiation the γ-H2AX foci were analyzed as described in Materials and Methods. (b) Representative image for the residual DSBs in cells transfected with control-siRNA and siRNA against different Akt isoforms 24 h after irradiation with 4 Gy. (c) Figures are presenting the mean numbers of foci/cell±S.E.M. from four independent replicates (800 nuclei) of the AKT1-siRNA experiment, three independent replicates (600 nuclei) of the AKT2-siRNA experiment and two independent replicates (400 nuclei) using the Akt3-siRNA (*P<0.05; ***P<0.001).

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