Figure 2
UnAG improves liver mitochondria respiration. (a) Histogram representation of oxygen consumption during State 3 (that is, ADP stimulated) respiration of liver mitochondria recovered from animals and treated as indicated (*P<0.05 UnAG+I/R versus I/R).Data are reported as mean±S.E.M. and representative of five different experiments, performed in triplicate (N=5). (b) Histogram representation of ADP/O ratio on liver mitochondria, treated as indicated; the absolute value reflects the nmoles of ADP phoshorylated per oxygen atom (*P<0.05 UnAG+I/R versus I/R). Data are reported as mean±S.E.M. and representative of five different experiments, performed in triplicate (N=5). (c) Histogram representation of oxygen consumption during post phosphorylative State 4 respiration on liver mitochondria, treated as indicated. Data are reported as mean±S.E.M. and representative of five different experiments, performed in triplicate (N=5). (d) Histogram representation of Respiratory Control Ratio (R.C.R.), the normalized index of ETC performance, which is obtained by dividing State 3 to State 4 (*P<0.05 UnAG+I/R versus I/R). Data are reported as mean±S.E.M. and representative of five different experiments, performed in triplicate (N=5). (e, f) Histogram representation of plasmatic concentration (International Units per liter) of the hepatic ALT (e) and AST (f) enzymes (the following both for ALT and AST: *P<0.05 UnAG+I/R versus I/R; *P<0.05 Ctrl versus UnAG+I/R and I/R). Data are reported as mean±S.E.M. and representative of five different experiments, performed in triplicate (N=5).
