Figure 4

UnAG upregulates Opa1 expression resulting in ATP production and apoptosis resistance during in vitro ischemia/reoxygenation (I/Reo). (a) Cell extracts from RNT cells subjected to in vitro ischemia/reoxygenation (I/Reo), treated as indicated, were analyzed for Opa1, CoxIV and Bcl-2 content, normalized to β actin content (for Opa1 and Bcl-2, *P<0.05 UnAG+I/Reo versus I/Reo; for CoxIV, *P<0.05 UnAG versus Ctrl and *P<0.05 UnAG+I/Reo versus I/Reo). Data are reported as mean±S.E.M. and representative of three different experiments, performed in triplicate (N=3). (b) Histogram representation of the relative cellular ATP content. Data are obtained from RNT cells, treated as indicated (*P<0.05 UnAG+I/Reo versus I/Reo). Data are reported as mean±S.E.M. and representative of four different experiments, performed in triplicate (N=4). (c) Apoptosis assay was performed on RNT cells subjected to I/Reo treated as indicated. Doxorubicin (1 μM) was used as a positive control (*P<0.05 UnAG+I/Reo versus I/Reo). Data are expressed as percentage±S.E.M. of total apoptotic cells, and representative of three different experiments, performed in triplicate (N=3).