Figure 6

UnAG impacts on Opa1-MIC60 interaction. (a) Representative co-immunoprecipitation (co-IP) of MIC60 from cell extracts (1 mg) of RNT cells treated as indicated, blotted with Opa1 and normalized to MIC60 (for MIC60, *P<0.05 UnAG+I/Reo versus I/Reo). (b) Representative co-IP of Opa1 from cell extracts (1 mg) of RNT cells, treated as indicated, blotted with MIC60 and normalized to Opa1 (*P<0.05 UnAG+I/Reo versus I/Reo, for MIC60 and Opa1). Data are reported as mean±S.E.M. and representative of four different experiments, performed in triplicate (N=4).