Figure 3
Development of resistance induced overexpression of Nrf2 and correlated proteins, an effect which was partially prevented by PN. MDA-MB231 cells were submitted to treatment with Mitox or DOX, as reported in methods, in order to develop the resistance. In some samples 2.0 μM PN were added two days before the drugs and maintained for the total selection time together with the drugs. Western blotting analysis was performed as reported in methods. (1) Parental cells; (2) cells selected with Mitox or DOX; (3) cells treated with Mitox or DOX plus PN. (a) Images of western blotting analysis. Results are representative of four different experiments. (b) Estimation by densitometric analysis of the intensity of the bands. The results are the means of four distinct experiments±S.D. *P<0.01 versus parental cells; #P<0.01 versus cells treated with Mitox or DOX plus PN.
