Table 1 Knockdown of Nrf2 prevented in MDA-MB231 cells the development of resistance induced by Mitox

From: Parthenolide prevents resistance of MDA-MB231 cells to doxorubicin and mitoxantrone: the role of Nrf2

Dose–response curve towards:

IC 50 values ( μ M)

RI values

 

Control siRNA

Nrf2 siRNA

Control siRNA (b/a)

Nrf2 siRNA (b/a)

 

a

b

a

b

  

PN

5.2±0.50

16.2±1.5

5.0±0.40

9.0±0.95

3.1±0.33

1.8±0.20

Mitox

0.7±0.08

1.4±0.15

0.9±0.08

1.0±0.10

2.0±0.20

1.1±0.12

DOX

0.8±0.08

1.6±0.16

0.9±0.08

1.2±0.12

2.0±0.22

1.3±0.15

  1. Abbreviations: a, parental cells; b, mitox-resistant cells; DOX, doxorubicin; Mitox, mitoxantrone; Nrf2, NF-E2-related factor 2; PN, parthenolide; RI, resistance index; siRNA, small interference RNA.
  2. Parental MDA-MB231 cells were transfected with Nrf2 siRNA or with control siRNA, as reported in Materials and Methods. After 24 h, the cells were submitted for 7 days to treatment with 10 nM Mitox in order to induce resistance. At the end, the cells were collected, counted and employed to test, in comparison with untreated cells, the effect exerted on cell viability by various doses of PN, Mitox and DOX. Dose–response curves (not shown) permitted to calculate IC50 and RI values reported in the Table. Results are the means±S.D. of three different experiments.
Back to article page