Figure 3
Autologous transplantation of monkey induced pluripotent stem cell (iPSC)-derived dopamine (DA) neurons and outcome analysis. (a) Magnetic resonance imaging (MRI)-guided stereotaxy and T2-weighted MRI at various time points after transplantation. MRI demonstrated that the cells had been accurately deposited into the target areas. (b, c) DAB staining for detection of TH+ cells and fibers (b, bʹ, bʹʹ: Parkinson's disease (PD) model with grafts; c,cʹ,cʹʹ: PD model without grafts) 6 months after transplantation. (d) Prussian blue staining to visualize transplanted cells in the lesioned side of striatum 6 months after transplantation. (e) The MRI signal areas of grafts over time. (f) The monkey engrafted with autologous iPSC-derived DA neurons showed behavioral recovery 6–8 weeks and 22–24 weeks after transplantation, whereas sham-grafted animals did not. Scale bars, b–d: 100 μm.
