Figure 4
Graft analysis. (a, b) Immunofluorescence staining for green fluorescent protein (GFP)- and TH-double positive cells in grafts 6 months after transplantation. (c-e) Immunofluorescence staining for detection of TH+ cells that co-expressed Foxa2 (c), Nurr1 (d) and GIRK2 (e), A9 region-specific marker, in grafts 6 months after transplantation. (f) Immunofluorescence staining for detection of TH+ cells that co-expressed Synaptophysin in grafts 6 months after transplantation. (g,h) Immunofluorescence staining for Iba1, Oct4, and Ki67 in grafts 6 months after transplantation. (i) Fractions of TH+ grafted cells that co-expressed Nurr1, Foxa2, or GIRK2 (at least 100 cells were analyzed). (j) Quantification of surviving TH+ cells in grafts at rostral striatum. (k– m) high-performance liquid chromatography (HPLC) analysis of dopamine (DA) and the metabolites (3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA)) at transplantation sites versus ipsilateral non-transplantation sites. Scale bars, a–h, 50 μm.
