Table 1 Summary of PTMs identified in this study

From: eIF2 interactions with initiator tRNA and eIF2B are regulated by post-translational modifications and conformational dynamics

Protein subunit

Modified residue

Highest Mascot score

TiO 2 enrichment

Direct analysis

Database

Conservation

eIF2α

N-term-ac-S2

46

 

×

+

 
 

pS194

16

×

 

+

 
 

pS292

24

 

×

A, B, C

S. pombe

 

pS294

17

 

×

A, B, C

S. pombe, C. elegans

 

pS301

42

 

×

B, C

C. elegans

eIF2β

N-term-ac-S2

63

 

×

+

 
 

pS40

76

×

×

A, B, C

S. pombe, C. albicans

 

acK89

49

 

×

+

 
 

pS91

39

×

 

C

S. pombe

 

pS92

43

×

×

A, C

S. pombe

 

pS112

64

×

×

A, B, C

D. melanogaster

eIF2γ

pT60

88

×

 

A, C

 
 

acK129

37

 

×

+

 
 

pT137

16

×

 

+

 
 

pS258

41

×

 

A, C

 
 

pS265

27

×

 

+

 
 

pS312

32

×

 

C

C. albicans

 

acK401

17

 

×

+

 
 

acK519

47

 

×

+

 

eIF2Bα

N-term-ac-S2

52

 

×

A

 
 

pY166

19

×

 

+

 

eIF2Bβ

N-term-ac-S2

79

 

×

+

 

eIF2Bγ

pS296

39

×

 

A, C

 
 

acK346

58

 

×

+

 

eIF2Bδ

pS131

49

×

 

+

 

eIF2Bε

pS435

66

×

 

C

 
 

pS449

72

×

 

+

 
 

pS525

67

×

×

A, C

H. sapiens

 

pS538

57

×

×

A, B, C

H. sapiens, M. musculus, S. pombe

  1. Abbreviations: ac, acetylation; eIF2, eukaryotic initiation factor 2; p, phosphorylation; PTMs, post-translational modifications; TiO2, titanium dioxide.
  2. Protein subunits of eIF2 and eIF2B and their modified residues (p and ac) with the highest observed Mascot score for each site are listed. It is indicated whether the sites were determined after TiO2 enrichment or by direct analysis. For PTMs that have been reported before the respective databases are given (A, UniProtKB; B, PHOSIDA; and C, PTMfunc).
  3. + Indicates novel sites. Note that acetylation sites have only been analysed without enrichment.
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