Figure 2 | Cell Discovery

Figure 2

From: Targeting the γ-/β-secretase interaction reduces β-amyloid generation and ameliorates Alzheimer’s disease-related pathogenesis

Figure 2

Screening of PS1/BACE1 interaction-interfering chemicals. (a) Representative results of the screen for chemicals that interfere with PS1-NTF-TEV/BACE1-CTEV interaction. Two hours after transfection, HEK293MSR cells were treated with 10 μM chemicals for 16 h and the luciferase reporter activities were measured. (b) PS1-NTF/BACE1 and KvBeta1/Kv1.1 split-TEV reporter activities. Following transfection, cells were treated for 16 h with 10 μM candidate compounds. (c) Cell viability of HEK293/APPswe cells. HEK293/APPswe were treated with 1 μM L-685 458, BACE1 inhibitor-IV (BSI-IV), BMS-708163, E2012 or 10 μM candidate compounds for 8 h and subjected to cell viability test. (d) BACE1 and (e) γ-secretase activity in fluorogenic substrate assay. HEK293T cell membrane fractions were incubated with 10 μM chemicals. (f) Total Aβ production of HEK293/APPswe cells. Cells were treated with 1 μM L-685 458, BSI-IV or BMS, or 10 μM candidate chemicals for 8 h. **P<0.01 determined by one-way ANOVA.

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