Figure 1
From: EZH2 promotes progression of small cell lung cancer by suppressing the TGF-β-Smad-ASCL1 pathway
TGF-β signal transduction is attenuated in several SCLC cells due to decreased expression of TβRII. (a and b) SCLC and NSCLC cells were stimulated with TGF-β for 2 h. (a) Immunoblot of cell lysates probed with the indicated antibodies; (b) qRT-PCR analysis of SMAD7 expression. Data represent mean±s.d. **P<0.01; ***P<0.001. (c) qRT-PCR analysis shows expression of TGF-β signaling components in SCLC and NSCLC cells. Data represent mean±s.d. (d) Comprehensive gene expression analysis from the NCBI GEO database (GSE32036) shows expression profiles of TGF-β signaling components in normal lung epithelial cells (n=59), SCLC cells (n=29) and NSCLC cells (n=119). Raw data were normalized by quantile algorithm. The color indicates the distance from the median of each row. GEO, gene expression omnibus; NCBI, National Center for Biotechnology Information; NSCLC, non-small cell lung cancer; SCLC, small cell lung cancer; TGF-β, transforming growth factor-β; qRT-PCR, quantitative real-time reverse transcription-PCR.
