Figure 5

Identification of the DNA motif bound by NAC050 and NAC052. (a) A palindromic DNA motif CTTGNNNNNCAAG identified in the binding regions of NAC050, NAC052, and JMJ14. (b) The binding assay using different amount of GST (glutathione S-transferase)-fused recombinant proteins showed significant binding affinity of NAC050 and NAC052 but not FYR (FYRN+FYRC) domain of JMJ14 (Jumonji C (JmjC) domain-containing protein 14) to the probe.