Figure 2
From: Increased dysbindin-1B isoform expression in schizophrenia and its propensity in aggresome formation
Differences in the subcellular distribution and solubility of dysbindin-1 isoforms and endogenous expression of human dysbindin-1B aggregates in mouse brain. (A) GFP-tagged dysbindin-1B forms aggregates in primary cultured cortical neurons (arrow). (B) The proportion of aggregate-containing neurons among those expressing GFP-tagged dysbindin-1A, -1B and -1C shown in panel E. Scale bars, 20 μm (panels A and C) and 5 μm (panel E). (C) A schematic diagram of a double LoxP system of human dysbindin-1B transgenic mouse model. (D) Endogenous expression of human dysbindin-1B in transgenic mouse is verified by western blot. (E) Endogenous expression of human dysbindin-1B in dysbindin1B+/−, CMV-Cre mouse formed aggregates in the cortex (arrows and arrowheads). Aggregate indicated by arrow is shown in Z-stack. Scale bars, 5 μm. (F) The solubility of myc-tagged dysbindin-1A, -1B and -1C in transfected HEK293 cells. Transfected cells were lysed with RIPA buffer. The same volume of soluble (s) and insoluble (i) fraction was subjected to immunoblotting with anti-myc antibody. (G) The proportion of soluble myc-tagged dysbindin-1A, -1B and -1C in the total lysates. Data are presented as mean±s.e.m. *P<0.05, ***P<0.001.GFP, green fluorescent protein.
